What are the Uses & Advantages of Micro-Injections

Micro-injection entails using a clear glass microneedle to inject foreign materials (e.g., DNA and RNA) and cells into biological targets such as an individual cell in culture, egg, embryo, or intracellular space. SeeTrue Needles is the advancement biomedical scientists need.

Advancing Medical Research

This thirty-year-old transgenic engineering technique has driven advances in fundamental research in

  • Cell & Systems Biology
  • Stem-cell Gene Manipulation
  • In Vitro Fertilization Cycle Therapy
  • Transgenic Biomedical Research

This technique has also allowed extensive human disease prevention modeling via pathophysiological research.

Improving Micro-injection Process

The manual micro-injection technique requires great practitioner skill, combining physical dexterity with sustained attention to detail. SeeTrue microcapillary needles improves the technical capabilities, reproducibility, and effectiveness of an important fundamental micro-injection process used in many areas of biomedical sciences.

Increasing Efficiency & Reproducibility

SeeTrue needle has the potential to increase the efficiency and reproducibility of manual micro-injection as well as automated visual-based robot-controlled injection.

Solving Deficiencies in Using the Current Needle

  • Visualize & Locate the Needle Outside and Inside the Injected Cell
  • Reduced Clogging With the SeeTrue Needles
  • Unified Needles Mean Calibration Time & Variability Are Greatly Reduced
  • Inner-needle Modifications to Eliminate Adherence of Cells Into the Inner Walls of the Microcapillary Needle
A Professional In Vitro Fertilisation Laboratory Microscope Closeup with circle background

Issues with the Current Industry-standard Microneedles (ISNs)

Industry-standard Micro-needles (ISNs) has four major deficits or customer pain-points that negatively impact experimental design, execution, repeatability, and overall efficacy:

  • Calibration Variability: The Manual Creation of Needle-tip Causes Undesired Variability
  • Susceptibility to Clogging: Cytoplasmic Material (E.g., Embryo Yolk) Can Clog the Needle-tip Opening
  • Low Contrast Visibility: The ISN’s Transparency Creates Low Contrast Visibility in Vitro, Leaving the User Unable to Track the Penetrating Needle-tip Leading to Missed-injection Targets and Needle Tip Breakage.
  • Adherence: Injected Cells Can Adhere to the Inner Walls of the Glass Capillary Needle, Leading to Costly Injected Material Loss (E.g., Sperm). This Forces You to Start All Over Again
  • Uncontrollable injection directionality: Because ISNs comprise a singular top opening, it is difficult or infeasible to inject substances in target directions distinct from that of insertion, such as in a single direction perpendicular to the insertion direction.
  • Tissue damage: Current microinjection techniques use steel or glass needles. Steel needles may cause tissue damage, as the inner and outer diameter of these needles is too large and, combined with the injected pressure applied, the tissue typically gets damaged. Glass ISNs may bend when lowered deeply into the brain, missing the target region.